Journal: Scientific Reports

Article Title: Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients

doi: 10.1038/srep10455

Figure Lengend Snippet: Structure of the surface of silver NHC chips with cultured cell samples by using a laser microscope. These are typical images using KATO-III. a, In ×100 view, many small and whitish nodules were recognized on the chip surface after adding a sample of chemically lysed cultured tumor cells. No nodules were observed after adding samples of extracted DNA ( d ) or RNA ( g ). After adding the extracted protein sample, small nodules and starch-like structures were observed ( j ). After adding a sample of physically fractured cultured tumor cells, many relatively large and whitish nodules were observed ( m ). In magnified (×3000 or ×1000) and 3D views, the largest nodule on the chip surface in ×100 view resembled a plain and hillocks (chemically lysed cultured tumor cells and extracted protein samples, b , c , k , l ) or a hill (physically fractured cultured tumor cells, n , o ). No on-chip structure was observed after adding samples of extracted DNA ( e , f ) or RNA ( h , i ).

Article Snippet: Kato III, MKN45, CW-2, and PK45-P were provided by the RIKEN Bioresource Center through the National Bio-Resource Project of the MEXT, Japan.

Techniques: Cell Culture, Microscopy, Starch